Our lab has two principal interests: the first, focuses on regulation of sodium transport in the collecting duct of the kidney which plays a major role in the regulation of volume homeostasis and hypertension. I study the transcriptional and post transcriptional regulation of the epithelial sodium channel, ENaC, and two proteins that interact with ENaC, a ubiquitin ligase Nedd4-2 and the kinase Sgk1. The second focuses on regulation of soluble Flt1, an anti- angiogenesis protein and the effect of soluble Flt1 on the glomerulus, where it appears to disrupt the glomerular capillary barrier and cause proteinuria. we have made several original observations about the genomic organization of Flt1, identified new alternate sFlt1 transcripts and described some aspects of the post transcriptional regulation of sFLT1 and of FLT1 ectodomain cleavage. Recently we described further regulation of FLT1 cleavage and reported that aspirin inhibits sFlt1 expression in trophoblasts.
Genetic Analysis of 400 Patients Refines Understanding and Implicates a New Gene in Atypical Hemolytic Uremic Syndrome.
Bu F, Zhang Y, Wang K, Borsa NG, Jones MB, Taylor AO, Takanami E, Meyer NC, Frees K, Thomas CP, Nester C, Smith RJH
J Am Soc Nephrol. 2018 Oct 30. pii: ASN.2018070759.
EGF regulation of proximal tubule cell proliferation and VEGF-A secretion.
Zepeda-Orozco D, Wen HM, Hamilton BA, Raikwar NS, Thomas CP.
Physiol Rep. 2017 Sep;5(18). pii: e13453. doi: 10.14814/phy2.13453. Epub 2017 Sep 27.
VEGF-A selectively inhibits FLT1 ectodomain shedding independent of receptor activation and receptor endocytosis.
Raikwar NS, Shibuya M, Thomas CP.
Am J Physiol Cell Physiol. 2018 Aug 1;315(2):C214-C224.
Late Reoccurrence of Collapsing FSGS After Transplantation of a Living-Related Kidney Bearing APOL 1 Risk Variants Without Disease Evident in Donor Supports the Second Hit Hypothesis.
Kalil RS, Smith RJ, Rastogi P, Katz DA, Thomas CP.
Transplant Direct. 2017 Jul 3;3(8):e185. doi: 10.1097/TXD.0000000000000697. eCollection 2017 Aug.
Aldosterone regulates a 5' variant sgk1 transcript via a shared hormone response element in the sgk1 5' regulatory region.
Raikwar NS, Thomas CP.
Physiol Rep. 2017 Apr;5(7). pii: e13221. doi: 10.14814/phy2.13221.
An Unexpected Surge in Plasma BKPyV Viral Load Heralds the Development of BKPyV-Associated Metastatic Bladder Cancer in a Lung Transplant Recipient With BKPyV Nephropathy.
Kuppachi S, Holanda D, Eberlein M, Alexiev B, Tyler AJ, Wissel MC, Kleiboeker SB, Thomas CP.
Am J Transplant. 2017 Mar;17(3):813-818. doi: 10.1111/ajt.14057. Epub 2016 Oct 21.
Familial C3 glomerulonephritis caused by a novel CFHR5-CFHR2 fusion gene.
Xiao X, Ghossein C, Tortajada A, Zhang Y, Meyer N, Jones M, Borsa NG, Nester CM, Thomas CP, de Córdoba SR, Smith RJ.
Mol Immunol. 2016 Sep;77:89-96. doi: 10.1016/j.molimm.2016.07.007. Epub 2016 Aug 1.
Has the Department of Veterans Affairs Found a Way to Avoid Racial Disparities in the Evaluation Process for Kidney Transplantation?
Freeman MA, Pleis JR, Bornemann KR, Croswell E, Dew MA, Chang CH, Switzer GE, Langone A, Mittal-Henkle A, Saha S, Ramkumar M, Adams Flohr J, Thomas CP, Myaskovsky L.
Transplantation. 2017 Jun;101(6):1191-1199. doi: 10.1097/TP.0000000000001377.